LOCUS       EF1a-RLuc-T2A-RF        7398 bp    DNA     linear        8-JUL-2013
SOURCE      
  ORGANISM  
            This file is created by Biosettia Inc
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     LTR             7164..7398
                     /label=3'\LTR
     intron          291..1455
                     /label=Intron
     LTR             1..181
                     /label=5'LTR
     misc_feature    6485..7081
                     /label=WPRE
     promoter        1698..3087
                     /label=human\EF1a\promoter
     intron          2126..3068
                     /label=intron
     CDS             5450..6475
                     /label=Hyg
     RBS             4842..5426
                     /label=IRES
     CDS             4079..4132
                     /label=T2A
     CDS             4133..4837
                     /label=RFP
     CDS             3134..4063
                     /label=Renilla\Luciferase\(RLuc)
BASE COUNT     1749 a      1900 c      2114 g      1635 t 
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca 
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc 
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta 
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa 
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc 
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc 
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg 
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc 
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga 
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca 
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg 
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg 
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac 
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc 
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg 
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt 
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga 
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa 
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt 
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta 
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca 
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata 
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga 
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga 
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa 
     1681 ttcaaaattt tatcgatgcc tccccgtcac cacccccccc aacccgcccc gaccggagct 
     1741 gagagtaatt catacaaaag gactcgcccc tgccttgggg aatcccaggg accgtcgtta 
     1801 aactcccact aacgtagaac ccagagatcg ctgcgttccc gccccctcac ccgcccgctc 
     1861 tcgtcatcac tgaggtggag aagagcatgc gtgaggctcc ggtgcccgtc agtgggcaga 
     1921 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc 
     1981 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt 
     2041 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg 
     2101 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct 
     2161 ctttacgggt tatggccctt gcgtgccttg aattacttcc acgcccctgg ctgcagtacg 
     2221 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt 
     2281 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg 
     2341 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt 
     2401 aaaatttttg atgatatcct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg 
     2461 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg 
     2521 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac 
     2581 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg 
     2641 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc 
     2701 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg 
     2761 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg 
     2821 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta 
     2881 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg 
     2941 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt 
     3001 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc 
     3061 catttcaggt gtcgtgaaaa ctacccctct agataatacg actcactata gggcccgggt 
     3121 tggatccgcc accatggctt ccaaggtgta cgaccccgag caacgcaaac gcatgatcac 
     3181 tgggcctcag tggtgggctc gctgcaagca aatgaacgtg ctggactcct tcatcaacta 
     3241 ctatgattcc gagaagcacg ccgagaacgc cgtgattttt ctgcatggta acgctgcctc 
     3301 cagctacctg tggaggcacg tcgtgcctca catcgagccc gtggctagat gcatcatccc 
     3361 tgatctgatc ggaatgggta agtccggcaa gagcgggaat ggctcatatc gcctcctgga 
     3421 tcactacaag tacctcaccg cttggttcga gctgctgaac cttccaaaga aaatcatctt 
     3481 tgtgggccac gactgggggg cttgtctggc ctttcactac tcctacgagc accaagacaa 
     3541 gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg atcgagtcct gggacgagtg 
     3601 gcctgacatc gaggaggata tcgccctgat caagagcgaa gagggcgaga aaatggtgct 
     3661 tgagaataac ttcttcgtcg agaccatgct cccaagcaag atcatgcgga aactggagcc 
     3721 tgaggagttc gctgcctacc tggagccatt caaggagaag ggcgaggtta gacggcctac 
     3781 cctctcctgg cctcgcgaga tccctctcgt taagggaggc aagcccgacg tcgtccagat 
     3841 tgtccgcaac tacaacgcct accttcgggc cagcgacgat ctgcctaaga tgttcatcga 
     3901 gtccgaccct gggttctttt ccaacgctat tgtcgaggga gctaagaagt tccctaacac 
     3961 cgagttcgtg aaggtgaagg gcctccactt cagccaggag gacgctccag atgaaatggg 
     4021 taagtacatc aagagcttcg tggagcgcgt gctgaagaac gaggctagcg gcgatatcga 
     4081 gggcagaggc agcctgctga catgcggaga tgtggaagag aaccccgggc ccatggtgtc 
     4141 caagggcgag gaagtgatca aagagttcat gcggttcaaa gtgcggatgg aaggcagcat 
     4201 gaacggccac gagttcgaga tcgagggcga gggcgaaggc agaccttacg agggaaccca 
     4261 gaccgccaag ctgaaagtga ccaagggcgg acccctgccc ttcgcctggg atattctgag 
     4321 cccccagttt atgtacggca gcaaagccta cgtgaagcac cccgccgaca tccccgacta 
     4381 caagaagctg agcttccccg agggcttcaa gtgggagaga gtgatgaact tcgaggacgg 
     4441 cggcctcgtg accgtgaccc aggatagctc tctgcaggac ggcaccctga tctacaaagt 
     4501 gaagatgcgg ggcaccaact tcccccccga tggccccgtg atgcagaaaa agaccatggg 
     4561 ctgggaggcc agcaccgaga gactgtaccc tagagatggc gtgctgaagg gcgagatcca 
     4621 ccaggccctg aagctgaagg atggcggcca ctacctggtg gagttcaaga ccatctacat 
     4681 ggccaagaaa cccgtgcagc tgcccggcta ctactacgtg gacaccaagc tggacatcac 
     4741 cagccacaac gaggactaca ccatcgtgga acagtacgag cggagcgagg gccggcacca 
     4801 cctgtttctg tatggcatgg acgagctgta caagtgaact agcccctctc cctccccccc 
     4861 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt 
     4921 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt 
     4981 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa 
     5041 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac 
     5101 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg 
     5161 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt 
     5221 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca 
     5281 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tacacatgct ttacatgtgt 
     5341 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg 
     5401 aaaaacacga tgataatatg gccacactag agatccaccg gtcgccacca tgaaaaagcc 
     5461 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga 
     5521 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg 
     5581 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta 
     5641 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag 
     5701 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc 
     5761 tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc 
     5821 ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata 
     5881 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac 
     5941 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg 
     6001 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt 
     6061 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga 
     6121 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca 
     6181 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc 
     6241 gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga 
     6301 tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt 
     6361 cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt 
     6421 actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg aataggcggc 
     6481 cgcgtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac 
     6541 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt 
     6601 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat 
     6661 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca 
     6721 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc 
     6781 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg 
     6841 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca 
     6901 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct 
     6961 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt 
     7021 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct 
     7081 ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc 
     7141 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc 
     7201 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg 
     7261 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag 
     7321 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag 
     7381 tgtggaaaat ctctagca 
//
